CIGAN ANDREW,FALCO SAVERIO CARL,GAO HUIRONG,LI ZHONGSEN,LIU ZHAN BIN,LYZNIK L ALEKSANDER,SHI JINRUI,SVITASHEV SERGEI,YOUNG JOSHUA K
申请号：
IN201617008551
公开号：
IN201617008551A
申请日:
2016.03.11
申请国别(地区):
IN
年份:
2016
代理人:
摘要：
Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism for gene editing and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
