The present invention discloses a method of combining pressure and enzyme to extract a hydrolysate of an animal-derived composition. The method comprises: performing a pre-process to an animal tissue to obtain a crude extract and adding an enzyme to hydrolyze the crude extract at a pressure of 0.2 ~ 400MPa, so as to obtain a hydrolyzed extract.本發明揭露一種結合壓力與酵素萃取動物性成份水解物之方法,該方法包含:對動物組織進行前處理步驟而獲得粗萃取物;以及將酵素加入粗萃取物中,並於0.2~400MPa之壓力下進行水解,進而取得水解萃取物。S1~S3‧‧‧步驟