PROBLEM TO BE SOLVED: To provide a method for determining a stress on bred rats easily and efficiently, and a material for a determination.SOLUTION: A method for determining a stress on mice based on a pH change in feces of bred rats is provided. A method for determining a stress on bred rats which uses a coloring reagent is provided. A material for a determination of a stress on bred rats which includes the coloring reagent is provided. A method for determining a stress on bred rats which uses the material for a determination of a stress on bred rats and a disposable cage equipped with the material for a determination of a stress is provided. As a result, the determination of the stress on the bred rats can be made more easily and efficiently.【課題】簡便に効率よく飼育ネズミのストレスを判定する方法及び判定材の提供。【解決手段】飼育ネズミの糞便のpH変化に基づくマウスのストレス判定方法;呈色試薬を用いる、飼育ネズミのストレス判定方法;呈色試薬を含む、飼育ネズミのストレス判定材;前記飼育ネズミのストレス判定材、前記ストレス判定材を備えるディスポーザブルケージを用いる飼育ネズミのストレス判定方法。これにより、より簡便に効率よく飼育ネズミのストレスを判定することが可能となる。【選択図】図1