The present invention is directed to a method for the detection of Borrelia burgdorferi. The invention also relates to the use of a composition in a method for the detection of Borrelia burgdorferi. The invention further relates to a composition which increases antibodies against Borrelia burgdorferi as well as increases the antigens of Borrelia burgdorferi. Alternatively, the invention relates to methods and means for counteracting, controlling or reducing the symptoms of Lyme's disease in a subject.