SOUCHE DE VIRUS ATTÉNUÉ DE LA FIÈVRE PORCINE AFRICAINE DÉVELOPPÉE DE MANIÈRE RATIONNELLE, PROTÉGEANT CONTRE UNE PROVOCATION PAR L'ISOLAT DU VIRUS PARENTAL DE GÉORGIE DE 2007
The United States of America, as represented by the Secretary of Agriculture;The University of Connecticut
发明人:
BORCA, Manuel V.,GLADUE, Douglas P.,HOLINKA-PATTERSON, Lauren G.,RISATTI, Guillermo R.,O'DONNELL, Vivian K.
申请号：
EP17821008
公开号：
EP3478705A4
申请日:
2017.06.26
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
African swine fever virus (ASFV) is the etiological agent of a contagious, often lethal viral disease of domestic pigs. The control of African Swine Fever (ASF) has been hampered by the unavailability of vaccines. Experimental vaccines have been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated ASFVs； however, these vaccines are only successful when protecting against homologous viruses. We have constructed a recombinant Δ9GL/ΔUK virus derived from the highly virulent ASFV Georgia 2007 (ASFV-G) isolate by deleting the specific virulence-associated 9GL (B119L) and the UK (DP96R) genes. In vivo, ASFV-G Δ9GL/ΔUK administered intramuscularly to swine even at relatively high doses (106 HAD50) does not induce disease. Importantly, animals infected with 104 or 106 HAD50 are solidly protected against the presentation of clinical disease when challenged at 28 days post infection with the virulent parental strain Georgia 2007.
