SOUCHE DE VIRUS ATTENUE DE LA FIEVRE PORCINE AFRICAINE DEVELOPPEE DE MANIERE RATIONNELLE, PROTEGEANT CONTRE UNE PROVOCATION PAR L'ISOLAT DU VIRUS PARENTAL DE GEORGIE DE 2007
THE UNITED STATES OF AMERICA; AS REPRESENTED BY THE SECRETARY OF AGRICULTURE;THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE;THE UNIVERSITY OF CONNECTICUT
发明人:
BORCA, MANUEL V.,GLADUE, DOUGLAS P.,HOLINKA-PATTERSON, LAUREN G.,RISATTI, GUILLERMO R.,O'DONNELL, VIVIAN K.,ODONNELL, VIVIAN K.
申请号：
CA3029552
公开号：
CA3029552A1
申请日:
2017.06.26
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
African swine fever virus (ASFV) is the etiological agent of a contagious, often lethal viral disease of domestic pigs. The control of African Swine Fever (ASF) has been hampered by the unavailability of vaccines. Experimental vaccines have been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated ASFVs； however, these vaccines are only successful when protecting against homologous viruses. We have constructed a recombinant ?9GL/?UK virus derived from the highly virulent ASFV Georgia 2007 (ASFV-G) isolate by deleting the specific virulence-associated 9GL (B119L) and the UK (DP96R) genes. In vivo, ASFV-G A9GL/AUK administered intramuscularly to swine even at relatively high doses (106 HAD50) does not induce disease. Importantly, animals infected with 104 or 106 HAD50 are solidly protected against the presentation of clinical disease when challenged at 28 days post infection with the virulent parental strain Georgia 2007.
