A cutting and slitting device comprises a base, a cutting mechanism, and a clamp. The cutting mechanism is mounted on the base and comprises a lower cutting wheel and an upper cutting wheel. The lower cutting wheel is mounted on the base and comprises a plurality of lower circular cutting plates. The upper cutting wheel is arranged above the lower cutting wheel and is in operative coupling with the lower cutting wheel. The upper cutting wheel comprises a plurality of upper circular cutting plates corresponding to the lower circular cutting plates. The clamp is mounted on the base in a manner of being movable toward the cutting mechanism and comprises an upper clamping member, a lower clamping member, and a plurality of slots. The upper clamping member is coupled to the lower clamping member to fix biological sensing substrates. The slots extend through the lower clamping member and the upper clamping member, and the slots respectively correspond to the upper circular cutting plates and the lower circular cutting plates to allow the upper circular cutting plates and the lower circular cutting plates to be respectively received in the slots.一種切割分條裝置包含一基座、一裁切機構及一夾具。裁切機構係設置於基座,並包含一下裁切刀輪具及一上裁切刀輪具,下裁切刀輪具係設置於基座上,並包含多個下圓刀切盤;上裁切刀輪具係設置於下裁切刀輪具上,並傳動連結於下裁切刀輪具,且上裁切刀輪具包含多個與下圓刀切盤相對應之上圓刀切盤。夾具係朝裁切機構可移動地設於基座,並包含一下夾具、一上夾具及多個開槽,上夾具係連結於下夾具,以固定生物感測基板;開槽係貫穿於下夾具與上夾具,且開槽係對應於上圓刀切盤與下圓刀切盤,以使上圓刀切盤與下圓刀切盤容置於相對應之開槽內。100‧‧‧切割分條裝置200‧‧‧生物感測基板1‧‧‧基座2‧‧‧裁切機構22‧‧‧上裁切刀輪具3‧‧‧夾具31‧‧‧滑台33‧‧‧上夾具34‧‧‧開槽L‧‧‧裁切方向