The present invention relates to a Bacillus amyloliquefaciens K317 strain suppressing the growth of antibiotics-resistant pathogenic microorganisms or enteropathogenic microorganisms, more precisely a Bacillus amyloliquefaciens K317 strain, a culture supernatant thereof and an antibacterial metabolite separated from the same. The Bacillus amyloliquefaciens K317 strain, the culture supernatant thereof and the antibacterial metabolite separated from the same of the invention can be effectively used for the composition, health food, and probiotics for inhibiting the growth of antibiotics-resistant pathogenic microorganisms and enteropathogenic microorganisms, since they have excellent antibacterial activity and inhibition effect on the growth of methicillin resistant pathogenic microorganism and such enteropathogenic microorganism as Edwardsiella tarda, E. coli and Staphylococcus epidermidis.
