An incision fixation device for single site laparoscopy is coupled with a sleeve to be positioned on a surgical incision. The sleeve has one isolation portion containing a passage. The fixation device includes a bracing member located in the passage, a compact member coupled to the isolation portion, and an upper lid containing a plurality of guiding portions and a second engaging portion. The bracing member has an anchor end and a first engaging portion. The compact member has at least one holding portion to hold the anchor end with the isolation portion interposed therebetween. The second engaging portion is releasably engaged with the first engaging portion. Each guiding portion has an instrument passage to guide a surgical instrument to pass through the surgical incision. The compact member has a first and a second fastening mechanisms that are movable against each other to press the anchor end and isolation portion.
