METHOD FOR GENE TRANSFER INTO PLANT BELONGING TO GENUS HORDEUM USING AGROBACTERIUM BACTERIUM, AND METHOD FOR PRODUCTION OF TRANSGENIC PLANT OF PLANT BELONGING TO GENUS HORDEUM
An object of the present invention is to provide a method of gene introduction, which can transform a Hordeum plant at a higher efficiency compared to that in known Agrobacterium methods, and a method of producing a transformed Hordeum plant. The method of the invention includes a step of subjecting an immature embryo tissue of a Hordeum plant to centrifugation treatment and/or pressurization treatment before the inoculation with Agrobacterium, during the coculture step, and/or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin b) containing a cytokinin and c) containing a phenoxy auxin in an amount of less than 2 µM and/or a benzoic auxin in an amount of less than 5 µM, or not containing any phenoxy auxin and/or benzoic auxin.
