THE SAMUEL ROBERTS NOBLE FOUNDATION;HARRISON, MARIA, J.;BURLIEGH, STEPHEN, H.;KARDAILSKY, IGOR
发明人:
HARRISON, MARIA, J.,BURLIEGH, STEPHEN, H.,KARDAILSKY, IGOR
申请号：
WO2000US10704
公开号：
WO0063400(A3)
申请日:
2000.04.20
申请国别(地区):
世界知识产权组织国际局
年份:
2001
代理人:
摘要：
A method of Agrobacterium-mediated genetic transformation using plants at the time of flowering has been found which is applicable to dicots and monocots capable of being transformed by Agrobacterium. The transformation method utilizes vacuum-infiltration to introduce the Agrobacterium T-DNA carrying a gene of interest into the plants, preferably plants grown from vernalized seed. Upon maturity, seeds collected from the infiltrated plants are germinated, and progeny carrying the transgene are selected. This transformation method produces progeny exhibiting stable inheritance of the transgene without the need for regeneration methods such as somatic embryogenesis or organogenesis.
