METHOD FOR GENE TRANSFER INTO PLANT BELONGING TO GENUS SORGHUM USING AGROBACTERIUM, AND METHOD FOR PRODUCTION OF TRANSGENIC PLANT OF PLANT BELONGING TO GENUS SORGHUM
PROBLEM TO BE SOLVED: To provide a method of gene introduction for a genus Sorghum plant and a method for producing a transformed plant, having higher efficiency than the Agrobacterium method.SOLUTION: This invention provides a gene introduction method of a genus Sorghum plant using a medium with an increased concentration of a nitrogen source and/or an increased concentration of inorganic ions selected from magnesium ions, potassium ions, calcium ion and sodium ion, in a step of preparing a plant tissue material of a genus Sorghum plant, a step of inoculation with Agrobacterium and/or a co-cultivation step. This invention also provides a method for producing a transformed plant using the gene introduction method.SELECTED DRAWING: NoneCOPYRIGHT: (C)2018,JPO&INPIT【課題】アグロバクテリウム法に比べて効率の高い、ソルガム属の植物の遺伝子導入方法および形質転換植物の作成方法の提供。【解決手段】ソルガム属の植物の植物組織材料を調製する工程、アグロバクテリウム菌を接種する工程及び／又は共存工程において、窒素源の濃度、ならびに／または、マグネシウムイオン、カリウムイオン、カルシウムイオンおよびナトリウムイオンから選択される無機イオンの濃度、を高めた培地を用いるソルガム属の植物の遺伝子導入方法。また、前記遺伝子導入方法を用いる形質転換植物の製造方法。【選択図】なし
