VariantsExpected vs Observed Frequenci. Expected Probability —Observed — — .00 5.00E-06 8.00 4.50E-06 7.00 4.00E-06 4?: 3.50E-06 6.00 3.00E-06 (5 5.00 2.50E-06 EL 4.00 2.00E-06 e-I 3.00 1.50E-06 2.00 1.00E-06 1.00 5.00E-07 0.00 0.00E+00 Ave rag e Coun t (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WIPO I PCT IiiimmommionotiolomolomollumioommovoimIE (10) International Publication Number WO 2018/170164 Al (51) International Patent Classification: C40B 30/06 (2006.01) C12N 5/071 (2010.01) C40B 40/08 (2006.01) C12N 5/10 (2006.01) C4OB 50/02 (2006.01) C12Q 1/68 (2018.01) C4OB 50/06 (2006.01) A61K 31/711 (2006.01) C12N 15/66 (2006.01) (21) International Application Number: PCT/US2018/022487 (22) International Filing Date: 14 March 2018 (14.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/471,723 15 March 2017 (15.03.2017) US 62/578,326 27 October 2017 (27.10.2017) US (71) Applicant: TWIST BIOSCIENCE CORPORATION [US/US]； 455 Mission Bay Blvd. South, Suite 545, San Francisco, California 94158 (US). (72) Inventors: COX, Anthony； 455 Mission Bay Blvd. South, Suite 545, San Francisco, California 94158 (US). CHEN, Siyuan； 455 Mission Bay Blvd. South, Suite 545, San Fran- cisco, California 94158 (US). LEDOGAR, Charles； 455 Mission Bay Blvd. South, Suite 545, San Francisco, Califor- nia 94158 (US). TOPPANI, Dominique； 455 Mission Bay Blvd. South, Suite 545, San Francisco, California 94158 (US). (74) Agent: HARBURGER, David； WILSON SONSINI GOODRICH & ROSATI, 650 Page Mill Road, Palo Alto, California 94304 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE,
