ABSTRACT Lyophilisation based process for the production of Bio fertilizers The present invention relates to the field of Bio fertilization. Particularly, the invention provides a lyophilisation based effective process for the production of lyophilised Bio- inoculants with enhanced shelf life along with other advantages. Present invention related to lyophilized bio-inoculants preparation that is an excellent shelf life to increase soil fertility and crop production using sustainable forming. Comparison analysis clearly showed that lyophilized bio-inoculants have a great shelf life potency to other conventional talc and liquid formulation. This process improve viabilities via suitable cryoprotectant and recipes design in lyophilisation steps. The lyophilisation based effective process for the production of lyophilised Bio-inoculants with enhanced shelf life, easy to field application in drip, drenching and flood irrigation system along with other advantages.