THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;UNIVERSITY OF WASHINGTON
发明人:
BAKER David,PULTZ Ingrid Swanson,SIEGEL Justin Bloomfield,STEWART Lance,TINBERG, Christine,WOLF Clancey
申请号：
TN2017000520
公开号：
TN2017000520A1
申请日:
2016.06.08
申请国别(地区):
TN
年份:
2019
代理人:
摘要：
Polypeptides, and methods for their use, are disclosed that have an amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO: 1, are provided, wherein (a) the polypeptide degrades a PFQPQLPY (SEQ ID NO: 140) peptide and/or a PFPQPQQPF (SEQ ID NO: 68) at pH 4； (b) residue 467 is Ser, residue 267 is Glu, and residue 271 is Asp； and (c) the polypeptide comprises an amino acid change from SEQ ID NO: 1 at one or more residues selected from the group consisting of 221, 262E, 268, 269, 270, 319A, 320, 354E/Q/R/Y, 358S/Q/T,368F/Q,399,402,406,424,449,461,463, 105, 171, 172, 173, 174,and 456.
