UNIVERSITY OF WASHINGTON;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人:
PULTZ, Ingrid, Swanson,WOLF, Clancey,SIEGEL, Justin, Bloomfield,TINBERG, Christine, Elaine,STEWART, Lance,BAKER, David
申请号：
PH12017502213
公开号：
PH12017502213A1
申请日:
2017.12.04
申请国别(地区):
PH
年份:
2018
代理人:
摘要：
Polypeptides, and methods for their use, are disclosed that have an amino acid sequence at least 75 pcnt identical to the amino acid sequence of SEQ ID NO: 1, are provided, wherein (a) the polypeptide degrades a PFQPQLPY (SEQ ID NO: 140) peptide and/or a PFPQPQQPF (SEQ ID NO: 68) at pH 4； (b) residue 467 is Ser, residue 267 is Glu, and residue 271 is Asp； and (c) the polypeptide comprises an amino acid change from SEQ ID NO: 1 at one or more residues selected from the group consisting of 221, 262E, 268, 269, 270, 319A, 320, 354E/Q/R/Y, 358S/Q/T, 368F/Q, 399, 402, 406, 424, 449, 461, 463, 105, 171, 172, 173, 174, and 456.
