UNIVERSITY OF WASHINGTON;The Regents of the University of California
发明人:
Ingrid Swanson PULTZ,Clancey WOLF,Justin Bloomfield SIEGEL,Christine Elaine TINBERG,Lance STEWART,David BAKER
申请号：
US16719183
公开号：
US20200109387A1
申请日:
2019.12.18
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Polypeptides, and methods for their use, are disclosed that have an amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:1, are provided, wherein (a) the polypeptide degrades a PFQPQLPY (SEQ ID NO: 140) peptide and/or a PFPQPQQPF (SEQ ID NO: 68) at pH 4； (b) residue 467 is Ser, residue 267 is Glu, and residue 271 is Asp； and (c) the polypeptide comprises an amino acid change from SEQ ID NO: 1 at one or more residues selected from the group consisting of 221, 262E, 268, 269, 270, 319A, 320, 354E/Q/R/Y, 358S/Q/T, 368F/Q, 399, 402, 406, 424, 449, 461, 463, 105, 171, 172, 173, 174, and 456.
