Richardson, Toby,Weiner, David,Luginbuhl, Peter,Solheid, Christopher,Gort, Steven John,Zhao, Lishan,McFarlan, Sara C.,Hicks, Paula M.,Burke, Ellen
申请号：
AU2007223913
公开号：
AU2007223913B2
申请日:
2007.03.07
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
This invention relates to polypeptides having aldolase activity, includingpyruvate activity such as, without limitation, HMG and/or KHG aldolase activity,polynucleotides encoding these polypeptides, and methods of making and usingthese polynucleotides and polypeptides. In some embodiments, the inventionis directed to polypeptides having aldolase activity, including pyruvate activitysuch as, without limitation, HMG and/or KHG aldolase activity, including thermostableand thermotolerant activity, and polynucleotides encoding these enzymes,and making and using these polynucleotides and polypeptides. The polypeptidesin accordance with the invention can be used in a variety of pharmaceutical, agriculturaland industrial contexts. In some embodiments, the invention provides polypeptidesand biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-ketoglutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,Rmonatin, and salts thereof, as well as certain stereoisomers of monatin derivatives,such as the R3R and S5R configurations, and salts thereof.
