The present invention provides the new injectable compositions comprising botulin toxin, can be administered to subject for various treatments, aesthetics and/or cosmetic purpose.The Injectable composition as included by the present invention shows the advantages of one or more Botoxs more than tradition, including reduced antigenicity, the tendency for undergoing undesired local diffusion after injection reduced, the efficiency of increased clinical efficacy duration in contrast or enhancing, the starting of faster clinical efficacy and/or improved stability.According to the present invention, the duration of effect of significant clinical response and at least six moon is provided in the subject through being subject to processing by the single treatment of the composition of injection, as provided by described processing method.