The invention relates to a urine collecting and sample kit, said kit comprising a self-standing urine collecting bag and an evacuated test tube. The collecting bag comprises a female connector fitment adapted to connect to a sample end of the evacuated test tube to enable taking a urine sample from urine deposited in the bag using the evacuated test tube. According to the invention, the female connector fitment is sealed by means of a sealing technique to a front and/or back panel of the self-standing urine collecting bag, at a height above a bottom panel of the bag, such that the insert opening of the fitment faces outward and the sample end of the test tube can be inserted into the recess and be removed from the recess while the bag is in its standing position.