A urine collection system adapted to be connected to a urinary catheter includes a drain tube and a collection bag. The collection bag has an inlet port and outlet port. The drain tube is positioned to communicate fluid from the catheter to the inlet port of the collection bag. A sampling port is provided which is in fluid communication with the drain tube upstream of the collection bag. In one embodiment, the sampling port is adapted to engage a slip-tip or luer lock type syringe. In another embodiment, the sampling port includes a flexible pierceable member. The urine collection system may also include an in-line vent positioned upstream of the collection bag. In yet another embodiment, the collection system includes a support member including vertically spaced mounting structures which are securable to the collection bag to facilitate mounting of the collection bag to a support structure at one of two vertically spaced positions.