PLANT BIOSCIENCE LIMITED;SEMINIS VEGETABLE SEEDS, INC.
发明人:
MITHEN, RICHARD F.,TRAKA, MARIA,BRUGMANS, BART W.
申请号：
CA2826720
公开号：
CA2826720A1
申请日:
2013.09.09
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
The present invention relates to a method for determining the genotype of aCruciferousvegetable plant for a plant with an increased glucosinolate level, comprisingobtaining asample of nucleic acids from said plant or a portion thereof and detecting insaid nucleicacids a polymorphism at the Myb28 locus that is genetically linked to anincreasedglucosinolate level. The polymorphism may comprises at least one of: a ) asinglenucleotide polymorphism (SNP) at a position corresponding to nucleotide 83,136, 226,563, 610, 830, 995, 1116, 1513, 1577, 1606, 1620, 1825, 1863, 1877 or 2026 ofSEQ IDNO: 1, or b) a polymorphism in the number of nucleotides present betweennucleotides323 and 332, between nucleotides 521 and 524, between nucleotides 783 and 786,between nucleotides and 909 and 914, between nucleotides 1365 and 1369,between1811 and 1821, or between nucleotides 2046 and 2056 of SEQ ID NO: 1, or c) apolymorphism in the number of nucleotides present between nucleotides 836 and837,between nucleotides 867 and 868, or between nucleotides 943 and 944 of SEQ IDNO:1.
