A manufacturing method of GUI LU ER XIAN JIAO comprises (A) adding at least one eatable acid to a mixture of tortoise shell and deerhorn to generate a herbal soup (B) physically vibrating the herbal soup to increase the extraction area of the tortoise shell and the deerhorn and the soluble rate of calcium contained in the herbal soup (C) separating the residual eatable acid from the herbal soup after being processed by the eatable acid to obtain a herbal extract (D) filtering the herbal extract to obtain a filtered herbal solution (E) cooking the filtered herbal solution (F) filtering the filtered herbal solution and (H) adding Chinese wolfberry and ginseng into the filtered herbal solution to further cooking to obtain GUI LU ER XIAN JIAO solution.一種龜鹿二仙膠製備方法,包含步驟:A)添加至少一種可食用酸至一龜板與鹿角藥材混合物中以產生一藥材湯液;B)利用一物理性震盪處理該藥材湯液,其係用以增加龜板以及鹿角藥材萃取面積與所含鈣質之溶出速率;C)分離業經可食用酸處理之該藥材湯液所含殘餘可食用酸而得到一藥材純液;D)過濾該藥材純液得到一藥材濾液;E)熬煮該藥材濾液;F)過濾該藥材濾液;以及H)添加枸杞與人參於該藥材濾液中一同熬煉而得到一龜鹿二仙膠原液。