WATANABE, Fumiaki,HARA, Ai,AMBO, Takanori,KITAHARA, Aya
申请号：
AU20150203203
公开号：
AU2015203203(B2)
申请日:
2015.06.15
申请国别(地区):
澳大利亚
年份:
2017
代理人:
摘要：
Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GXiX 2X3X4 DX 5 X6 R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine. - 61 -
