There is provided a technology for simple three-dimensional culturing of cartilage cells. The technology includes a method for culturing cartilage cells which comprises seeding cartilage cells 1 on a plurality of projected portions 21 of a culturing substrate 2 having a culture surface on which the projected portions 21 having an equivalent diameter and an interval which are smaller than the equivalent diameter of the cartilage cells 1 to be cultured, placing the culture substrate 2 having the cartilage cells 1 in a culture vessel, and culturing the cartilage cells to form aggregates of cartilage cells.
