MERCK SHARP & DOHME CORP.;INTERVET INTERNATIONAL B.V.
发明人:
HEINRICHS JON HENRY,BODMER JEAN LUC,SECORE SUSAN LYNN,GOERKE AARON RUDY,CARO AGUILAR IVETTE,GENTILE MARIE PIERRE,HORTON MELANIE S.,MIEZEIEWSKI MATTHEW RYAN,SKINNER JULIE M.,SONDERMEIJER PAULUS JACOBUS
申请号：
IN4187CHN2014
公开号：
IN4187CHN2014A
申请日:
2014.06.04
申请国别(地区):
IN
年份:
2015
代理人:
摘要：
difficiledifficiledifficiledifficileThe present invention relates to recombinant C. toxin A (TcdA) and toxin B (TcdB) and binary toxin A (CDTa) proteins comprising specifically defined mutations relative to the native toxin sequence that substantially reduce or eliminate toxicity. The invention also relates to vaccines and immunogenic compositions comprising these recombinant toxins as well as combinations of these toxins with binary toxin B (CDTb) which are capable of providing protection against C. infection and/or the effects thereof. The invention also relates to methods of inducing an immune response to C. comprising administering the vaccines and immunogenic compositions described herein to a patient. The invention also encompasses methods of expressing recombinant C. toxin A and toxin B and CDTa mutants and CDTb in recombinant expression systems. In exemplary embodiments TcdA TcdB and CDTa mutant toxins comprising sufficient mutations to substantially reduce or eliminate toxicity are expressed in the baculovirus/insect cell expression system.
