A high throughput method to determine compounds that alter appetite, satiation or digestion in vertebrates is provided. In one embodiment a method to assay a test compound in a teleost larvae or adult is disclosed, comprising: providing a plurality of teleost larvae or adults in wells of a multi-well substrate having a food source comprising an optically detectable, ingestible, lipophilic molecule and a test compound, wherein at least one well has an individual larvae or adult simultaneously exposing the plurality of larvae or adults to a non- laser infrared light source and simultaneously detecting or quantifying emissions from the molecule while the teleost larvae or adult is alive and freely moving. Also disclosed, in one embodiment, is a method to detect nutrient homeostasis in a zebrafish larvae or adult, comprising: providing a plurality of live zebrafish larvae or adults in individual wells of a multi-well substrate having a food source comprising an optically detectable, ingestable, lipohilic molecule and detecting simultaneously with an infrared macroscope the presence and/or amount of the optically detectable, lipophilic molecule in the intestine of the live, freely moving zebrafish larvae or adult, thereby detecting or quantifying intake and/or digestion rates.Linvention concerne un procédé à haut rendement pour déterminer des composés qui modifient lappétit, la satiété ou la digestion chez les vertébrés. Linvention concerne un procédé de dosage dun composé dessai chez un téléostéen à létat larvaire ou adulte, comprenant les étapes consistant à : placer une pluralité de téléostéens à létat larvaire ou adulte dans des puits dun substrat multi-puits comportant une source alimentaire comprenant une molécule lipophile, ingérable, optiquement détectable, et un composé dessai, au moins un puits comprenant un individu à létat larvaire ou adulte exposer simultanément la pluralité de larves ou dadultes à une source de lumière infrarouge non laser et détecter ou qu
