Michael James Moser,David J. Marshall,James R. Prudent,Cristopher V. Van Hout,Christine A. Larsen
申请号：
US12884575
公开号：
US08313932B2
申请日:
2010.09.17
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.
