Methods for amplification of nucleic acids utilizing hairpin loop or duplex primers

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专利权人:

Arnold Lyle J.

发明人:

Arnold Lyle J.

申请号：

US201614999978

公开号：

US2017009282(A1)

申请日:

2016.07.21

申请国别(地区):

美国

年份:

2017

代理人:

摘要：

The present invention provides methods of amplifying a target nucleic acid utilizing duplex primer. The first strand of the primer comprises a random nucleotide sequence of about 6 to about 9 nucleotides in length that is able to hybridize to the target nucleic acid and a tag sequence. The second strand of the primer comprises a sequence complementary to the tag sequence allowing the primer to form a duplex and the ability to bind the tag sequence of the product nucleic acid for further amplification. The resulting nucleic acid produced contains tag sequences on both the 3′- and 5′-termini.

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