Disclosed herein is a structure for multi-stage sealing of a turbine, including: an annular multi-stage sealing part disposed on an inner circumferential surface of a fixed body and having different heights of at least two stages; a barrier unit disposed at a central side of the multi-stage sealing part on the inner circumferential surface of the fixed body; and an annular multi-stage tooth part disposed to face the multi-stage sealing part or the barrier unit over an outer circumferential surface of a rotating body and provided with a plurality of teeth, having a relative height corresponding to different heights of the multi-stage sealing part or the barrier unit at an interval between the fixed body and the rotating body.