The present invention provides an extraction method of collagen solution from tuna connective tissue. Collagen is pretreated by sodium phosphate, alkaline solution or alkaline metal hydroxide solution, ethanol solution and extracted by organic acid solution and enzyme, and the odor of collagen is removed by activated carbon filtration device. The present invention further provides a product which is used in biomaterial and cosmetics, comprising collagen solution from tuna.本發明提供一種鮪魚膠原蛋白溶液之萃取方法,其利用磷酸鹽、鹼性溶液或鹼金屬氫氧化物溶液及醇類溶液進行前處理,並以有機酸溶液及酵素萃取膠原蛋白,並將該膠原蛋白利用活性碳過濾裝置去除異味。本發明進一步提供一種用於生醫材料或化妝品之製品,其包含鮪魚膠原蛋白溶液。10...本發明之萃取膠原蛋白流程圖101...鮪魚前處理102...磷酸鹽溶液處理103...鹼性溶液或鹼金屬氫氧化物溶液處理104...醇類溶液處理105...以酸溶液/酵素進行106...獲得膠原蛋白溶液