The invention relates to a process of preparing low molecular weight peptides from gelatine. The gelatine is broken down into low molecular weight peptides using enzymes from Bacillus sp. to obtain peptides with low ash content. The gelatine is clarified by adjusting the pH to 7 using alkali hydroxide and filtered. The filtered solution is desalted at 20 to 55° C. in a diafiltration mode till the salt content decreases below 0.05% (w/v) The product so obtained is hydrolysed with an enzyme maintaining pH in the range of 7 to 10 and at temperature in the range of 25 to 75° C. The hydrolysed product is filtered through ultrafiltration membrane at temperature in the range of 15 to 55° C. and permeate is concentrateds at temperature in the range of 50 to 100° C. under vacuum to obtain the peptide solution of the invention.
