expression construct for transforming a unicellular host, unicellular host cell as well as a method of producing preprolisostafine and prolisostafine free mature lysostafin endopeptidase

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专利权人:

发明人:

GHAN SHYAM KHATRI,RAHUL SHARMA

申请号：

BR9917526

公开号：

BR9917526B1

申请日:

1999.10.19

申请国别(地区):

BR

年份:

2013

代理人:

摘要：

A portion of the lysostaphin gene of Staphylococcus simulans has been cloned and overexpressed in the cytoplasm of E. coli to yield lysostaphin, in the absence of preprolysostaphin and prolysostaphin, under the transcriptional control of an IPTG-inducible promoter and a ribosome binding site. IPTG induction of the transformed host cells produces intracellular, soluble, mature lysostaphin (27 kDa), in the complete absence of preprolysostaphin and prolysostaphin. The mature lysostaphin so formed dose not require post-translational modification. The mature lysostaphin so formed can be used treat and prevent staphylococcal infections.

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中国工程科技知识中心

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