DE KREIJ, ARNO,MADRID, SUSAN MAMPUSTA,MIKKELSEN, JOERN DALGAARD,SOEE, JOERN BORCH
申请号：
ES06014355
公开号：
ES2375492T3
申请日:
2004.01.15
申请国别(地区):
ES
年份:
2012
代理人:
摘要：
Use of a lipid acyltransferase to prepare from a food material containing water comprising 10-98% water a food selected from egg or an egg-based product or a dairy product comprising an emulsifier, in which the Emulsifier is generated from constituents of the food material by the lipid acyltransferase, in which the lipid acyltransferase is one that when tested using the transferase assay in buffered substrate exhibits an acyltransferase activity of at least 2%； said transferase assay comprising the steps consisting of: i) dissolving 450 mg of phosphatidylcholine and 50 mg of cholesterol in chloroform, evaporating to dryness in vacuo； ii) transfer 300 mg of the cholesterol / phosphatidylcholine mixture to a Wheaton glass, add 15 ml of 50mM HEPES buffer pH 7 and disperse the lipid in the buffer during stirring； iii) heat the substrate to 35 ° C during mixing with a magnetic stirrer and add 0.25 ml of enzymatic solution； iv) take 2 ml samples at the reaction time of 0, 5, 10, 15, 25, 40 and 60 minutes and immediately interrupt the enzymatic reaction by adding 25 l of 4M HCl to acidify the free fatty acid； v) add 3 ml of chloroform and shake vigorously for 30 seconds, centrifuge and isolate 2 ml of the chloroform phase, filter through a 0.45 µl filter in a 10 ml tarred Dram vessel； vii) evaporate the chloroform under a stream of nitrogen at 60 ° C, and weigh the samples； viii) analyze the lipid extracted by GLC.Utilización de una lípido aciltransferasa para preparar a partir de un material alimenticio que contiene agua que comprende agua al 10-98% un alimento seleccionado de entre huevo o un producto a base de huevo o un producto lácteo que comprende un emulsionante, en la que el emulsionante es generado a partir de constituyentes del material alimenticio por la lípido aciltransferasa, en la que la lípido aciltransferasa es una que cuando es sometida a prueba utilizando el ensayo de transferasa en sustrato tamponado presenta una actividad de aciltransferasa de por lo me
