Arizona Board of Regents on behalf of Arizona State University
发明人:
Deirdre Meldrum,Fengyu Su,Laimonas Kelbauskas,Vivek Nandakumar,Yanqing Tian,Roger Johnson
申请号：
US16631040
公开号：
US20200178967A1
申请日:
2018.08.29
申请国别(地区):
US
年份:
2020
代理人:
摘要：
A thixotropic and biocompatible gel, formed by combining of 4-arm polyethylene glycol with fumed silica (PEG-silica). The thixotropicity of the gel is affected by the ratio of PEG to silica. To study the gels biocompatibility, the cell suspension in PBS or another medium was added to the PEG-silica gel via mixing to uniformly disperse the cells in the gel and then placed in an incubator before performing fluorescence live-dead assays. Duration of cell viability (cell life time) in gels was measured to be up to several days, depending on gel composition. Due to their optical and fluidic properties the gels are compatible with live cell imaging including 3D computed tomography and offer a means for moving the cells in a highly controllable manner by applying and removing pressure on the gels.
