The Simple and rapid methods for estimation of milk protein. The present invention describes about the clarifying agents for rapid milk clarification for the analysis of milk quality by measuring the milk protein. Propionic acid: Iso propanol: Water (5:4:1) cocktail solution is developed for instant milk clarification and without affecting the absorption property of milk protein at 280nm. Second clarification agent, n-Butylamine: n-Butanol (4:1) specially developed for clarification of milk in one minute for non-interference of estimation of milk protein based on Biuret colorimetric method. Clarification mediated estimation of milk protein is reliable for true protein measurement, not affected by the adulterants such as water, sugar, salt, oil and urea and it can be adapted to high-throughput format and simple optical instrumentation for field level application.
