A method of preparing microsphere containing hydroxylapatite and gelatin is applicable to biomedical composite technology, mainly comprising using hydrothermal method to add ammonium dihydrogen phosphate and calcium nitrate in gelatin solution and preparing microsphere under proper heating temperature, stirring time, and stirring rate. The preparation method of this invention does not require any organic solvent, capable of avoiding residue of toxic chemicals in addition, pH value adjustment or adding crosslinking agent is also not required, and the microsphere containing hydroxylapatite and gelatin can be produced by controlling a specific temperature range only.一種具氫氧基磷灰石及明膠的微米球製備方法,係應用在生醫複合技術中,主要技術在於利用水熱法在明膠水溶液中加入磷酸二氫氨和硝酸鈣,並在適當的加熱溫度、攪拌時間及攪拌速率下製備生成微米球,本發明之製備方法不需任何有機溶劑,可以避免毒性化學物質的殘留,亦不需調整pH值或加入交聯劑,只需控制在特定溫度範圍下即可生成具氫氧基磷灰石及明膠的微米球。S1~S9‧‧‧步驟