PROCÉDÉ POUR PRODUIRE, ISOLER ET PURIFIER DE L'ANTITRYPTASE HUMAINE RECOMBINANTE (OSRAAT) À PARTIR DE SEMENCES DE RIZ

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专利权人:

WUHAN HEALTHGEN BIOTECHNOLOGY CORP

发明人:

YANG, DAICHANG,SHI, QIANNI,ZHANG, LIPING

申请号：

EP13853496

公开号：

EP2918680A4

申请日:

2013.04.26

申请国别(地区):

EP

年份:

2016

代理人:

摘要：

Provided in the present invention are a rice genetic codon-optimized OsrAAT gene, related vector and a method for expression of OsrAAT in transgenic rice seeds by using the vector and a method for isolating and purifying OsrAAT. A transgenic rice seed expressing OsrAAT is prepared by the endosperm-specific expression vector. The purity of OsrAAT isolated and purified from transgenic rice grains reached 97% with HPLC grade and the yield of OsrAAT was up to 18.89 ± 3.19% of total soluble proteins, thus corresponding that 0.366 g of OsrAAT per kilogram can be produced from brown rice.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/