PROCEDE POUR PRODUIRE, ISOLER ET PURIFIER DE L'ANTITRYPTASE HUMAINE RECOMBINANTE (OSRAAT) A PARTIR DE SEMENCES DE RIZ

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专利权人:

WUHAN HEALTHGEN BIOTECHNOLOGY CORP

发明人:

YANG, DAICHANG,SHI, QIANNI,ZHANG, LIPING

申请号：

CA2890659

公开号：

CA2890659A1

申请日:

2013.04.26

申请国别(地区):

CA

年份:

2014

代理人:

摘要：

Provided in the present invention are a codon optimized OsrAAT inherited rice gene, related vector and method for preparing OsrAAT transgenic rice seeds and method for isolating and purifying OsrAAT. OsrAAT transgenic rice seeds are prepared by the specific expression of the expression vector of OsrAAT through the rice albuminous cell, from which OsrAAT is isolated and purified, the obtained OsrAAT having an HPLC purity of 97% and a yield of up to 18.89 ± 3.19%, thus corresponding to being able to produce 0.366 g of OsrAAT per kilogram of unrefined rice.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/