DOSHI, KETAN M.,LOUKANINA, NATALIA N.,POLLOCK, BRENT,POLOWICK, PATRICIA,HOLBROOK LARRY
申请号：
CA2830548
公开号：
CA2830548A1
申请日:
2013.10.17
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
The present disclosure describes reproducible methods for the Agrobacterium-mediatedproduction of stable, genetically transformed, fertile tarwi plants (Lupinusmutabilis Sweet)having seed-specific expression of human adenosine deaminase enzyme (hADA) orafunctional variant thereof. The method involves slicing a tarwi seed embryo toproduce anexplant infecting the explant in co-cultivation medium containing anagrobacterium having apolynucleotide sequence encoding hADA or variant thereby generating atransformedexplant elongating a transformed shoot from the transformed explant andregenerating atransformed tarwi plant from the elongated shoot. Seeds and plants so formedare alsodescribed herein. Further, methods for the recovery and purification ofrecombinant hADA, orfunctional variant from a transformed tarwi plant are described.
