A method for manufacturing a biomedical signal conductive membrane includes providing a natural polymer material, adding an acid solution for making the natural polymer material dissolved in the acid solution to form a viscous liquid mixture, performing a dry step for drying the viscous liquid mixture to form an initial stage of membrane, wherein the structure of natural polymer material within the initial stage of membrane is a straight chain, providing a cross-linking agent to perform a cross-linking step for transforming the initial stage of membrane into a gel membrane, wherein the structure of natural polymer material within the gel membrane is a cross-linked network, and performing a rinse step for removing the cross-linking agent and keeping a plurality of water molecules remaining within the cross-linked network of natural polymer material.