An endoscopic diagnosis system includes a light source for simultaneously emitting at least two excitation light having different central wavelengths for exciting at least two autofluorescent substances to generate autofluorescence containing first autofluorescence whose intensity is strong in a lesion and weak in a normal region and second autofluorescence whose intensity is strong in a normal region and weak in a lesion, color filters having spectral transmission characteristics that block the excitation light and transmit the autofluorescence, image sensor for imaging autofluorescence generated from first and second autofluorescent substances contained in a subjects region under observation and dispersed by color filters to acquire first and second autofluorescence images as the at least two excitation light illuminate the region under observation, and an image sensor for image-processing and displaying the first and second fluorescence images.
