Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Kubanskij gosudarstvennyj agrarnyj universitet imeni I.T. Trubilina"
发明人:
Potapova Anna Yurevna,Serdyuk Ivan Yurevich,Dzhailidi Georgij Anastasovich,Chernykh Oleg Yurevich,Krivonos Roman Anatolevich,Koshchaev Andrej Georgievich,Lysenko Aleksandr Anatolievich,Shevchenko Aleksandr Alekseevich
申请号：
RU20170111554
公开号：
RU2645263(C1)
申请日:
2017.04.05
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: veterinary medicine.SUBSTANCE: invention relates to the field of veterinary virology, in particular to a test system for detecting the DNA of a particularly dangerous agent of African swine fever (ASF). Test system for detecting DNA of the African swine fever virus using real-time polymerase chain reaction includes plastic vials and test tubes, a thermostable Tag-polymerase enzyme, a buffer for the reaction, a mixture of four deoxynucleotide triphosphates, a positive control – recombinant plasmid containing a fragment of the vp72 gene of the African swine fever virus, synthetic oligonucleotide primers and a probe. Synthetic oligonucleotide primers and a fluorescent probe complementary to the preserved region of the genome of the African swine fever virus gene vp72 region are used and have the following nucleotide composition: SEQ NO 1: 5’-CTG-CTC-ATG-GTA-TCA-ATC-3’ – forward primer, SEQ NO 2: 5’-GAT-ACC-ACA-AGA-TCG-CCG-3’ – reverse primer, SEQ NO 3: 5’-FAM-CCA-CGG-GAG-GAA-TAC-CAA-
