An extract of Antrodia solid culture mycelium and its use in anti-metastasis of lung cancer cells are disclosed the extract of Antrodia solid culture mycelium is 1,2,4-trimethoxy-6-methylbenzene-3-ol, also known as Leader 1 said Leader 1 can effectively inhibit the movement, migration and invasion ability of lung cancer cells, and thus achieve the effect of anti-metastasis of lung cancer cells it is capable of exhibiting excellent physiological activity and mechanism of anti-metastasis of lung cancer cells.一種樟芝固態培養菌絲體之萃取物及其抗肺癌細胞轉移之用途,該樟芝固態培養菌絲體之萃取物為1,2,4-Trimethoxy-6-methylbenzene-3-ol,又稱利得1號(Leader 1),該Leader 1能有效抑制肺癌腫瘤細胞之運動、遷徙及侵襲之能力,進而達到抗肺癌細胞轉移之效果,可展現優異之抗肺癌細胞轉移之生理活性與作用機制。