PURPOSE: A method for removing pigment of red beet and enhancing physiological activity by radiation is provided to be used in a cosmetic, medicinal, or health food composition. CONSTITUTION: A method for removing pigment and maintaining physiological activity of red beet extract comprises a step of irradiating radiation to the red beet extract. A method for enhancing anti-wrinkling activity of the extract comprises a step of irradiating radiation to the extract. The extract is prepared by water, alcohol or mixture thereof. The alcohol includes low alcohol of C1-C4. The radiation includes gamma ray, electronic beam, UV or X ray. The pigment includes betanin or isobetanine. A cosmetic composition for anti-wrinkling, skin whitening, or antioxidation contains the red beet extract as an active ingredient.본 발명은 방사선을 이용한 레드비트 추출물의 색소를 제거하는 방법 또는 생리활성 증진 방법에 관한 것으로서, 본 발명의 방사선이 조사된 레드비트 추출물은 생리 활성은 증진시키고, 복잡한 색소 제거 과정 없이 레드비트 추출물의 색소를 제거할 수 있으므로 화장료 조성물, 의약품 조성물 또는 건강식품 조성물로 유용하게 사용할 수 있다.