Kozlov Vladimir Aleksandrovich,Kolesnikova Olga Petrovna,Demchenko Elena Nikolaevna,Gojman Elena Vladimirovna,Gavrilova Elena Davidovna
申请号：
RU20180111883
公开号：
RU2679320(C1)
申请日:
2018.04.02
申请国别(地区):
俄罗斯
年份:
2019
代理人:
摘要：
FIELD: veterinary medicine.SUBSTANCE: invention relates to a method for predicting systemic inflammatory conditions in an animal that can lead to the development of nephritis in an induced model of an autoimmune disease of systemic lupus erythematosus. Method of early prediction of nephritis development in an induced model of an autoimmune disease of systemic lupus erythematosus includes administering a dose of lipopolysaccharide to healthy animals, followed by a simultaneous assessment of the level of inflammation in the blood cells and the level of extracellular DNA in the blood plasma. Lipopolysaccharide is intraperitoneally administered to healthy hybrids (C57BL/6 × DBA2)F1 at a dose of 50 mcg/kg, then for 2, 4, 8, 11 and 24 hours from the moment of its administration in the dynamics, the index of the ratio of neutrophils to lymphocytes (N/L) and extracellular DNA is evaluated in the blood and 24 hours after the administration of lipopolysaccharide a systemic lupus erythematosus model is induced by intra
