PROCESS FOR GENOTYPIFICATION AND SELECTION OF BOVINE CATTLE FOR THE PRODUCTION OF BETA-CASEINE A1 FREE COW MILK AND ITS USE IN THE PREPARATION OF FOOD FOR CHILDRENS NUTRITION.
Jorge Luis ROSADO LORIA,Carlos Raúl GARCÍA UGALDE,Miguel Angel DUARTE VÁZQUEZ
申请号：
MX2017008445
公开号：
MX2017008445A
申请日:
2017.22.06
申请国别(地区):
MX
年份:
2019
代理人:
摘要：
The present disclosure is related to the biotechnological industry and the specialized food manufacturing industry. The advantage of the present invention with respect to the compositions of the state of the art, lies in its ability to genotype cattle according to the CSN2 gene to identify and select bulls and cows that bear the genotype CSN2 A2 / A2. To reproduce and raise dairy cattle that have the capacity to produce milk composed of BCAS A2 and that is free of BCAS A1. Cows milk with the aforementioned characteristics can be used for manufacturing specialized products for infant feeding. The present method involves the insertion of a point mutation in the codon, where the bovine polymorphism of the BCAS A1 and the BCAS A2 occurs, using a modified oligonucleotide at its 5 end. The product of the CSR PCR, which results from the amplification of CSN2 A2, presenting a restriction site for a specific endonuclease, which does not occur with the product of the CSR PCR of CSN2 A1. After the amplification, the products of the CSR PCR are digested with the specific endonuclease and the restriction fragments are analyzed on a 1% of agarose gel. The genotype of the animals is defined according to the pattern of the fragment bands, which result from the restriction enzymatic digestion.La presente invención está relacionada con la industria biotecnológica y con la industria de la manufactura de alimentos especializados. La ventaja de la presente invención con respecto de las composiciones del estado de la técnica, radica en su capacidad para genotipificar el ganado bovino en función del gen CSN2 para identificar y seleccionar los toros y las vacas que portan el genotipo CSN2 A2/A2, para reproducirlos y criar ganado bovino lechero que tenga la capacidad de producir leche compuesta de la BCAS A2 y que esté libre de la BCAS A1. La leche de vaca con las características mencionadas podrá ser utilizada en la fabricación de productos especializados para la alimentación de infantes.
