A microwave imaging method and a microwave imaging system and a bone-assessment method using the same are provided. The microwave imaging method includes following steps: emitting a plurality of microwave signals with different frequency receiving a plurality of scattered field data generated based on the microwave signals calculating a dielectric constant distribution of an object-under-test under the corresponding frequency according to each of the scattered field data, where the dielectric constant distributions corresponding to the different frequency respectively have different analytical depth generating a corresponding tomography data according to each of the dielectric constant distributions and building a three-dimensional tomography of the object-under-test according to the tomography data.一種微波成像方法及應用其之成像系統與骨質評估方法。所述微波成像方法包括以下步驟:發射具有不同頻率的多個微波訊號;接收基於所述多個微波訊號所產生之多個散射場資料;依據各個散射場資料計算待測物體在對應的頻率下的介電係數分佈,其中對應於不同頻率的介電係數分佈分別具有不同的解析深度;依據各個介電係數分佈產生對應的斷層影像資料;以及基於多個所述斷層影像資料建立待測物體的三維斷層影像。S210~S250‧‧‧步驟