A method and system for imaging a tissue having contrast agents dispersed therein by exciting nonlinear response of the contrast agents using dual-frequency ultrasound, including transmitting a dual-frequency ultrasound to a target zone having the contrast agents dispersed therein, both frequencies of the dual-frequency ultrasound being higher than a resonance frequency of the contrast agents, and a frequency difference between the frequencies of the dual-frequency ultrasound is within a predetermined range surrounding the resonance frequency of the contrast agents.一種用於組織之成像方法及系統,該組織具有一對比劑擴散在其中,藉由使用雙頻超音波激發該對比劑之非線性響應,該成像方法及系統包含發射一雙頻超音波至一目標區,其中該目標區具有該對比劑擴散在其中,該雙頻超音波之兩種頻率皆高於該對比劑之共振頻率,且該雙頻超音波之兩種頻率間的頻率差介於該對比劑之共振頻率附近的一預定範圍內。