BIEMANS RALPH LEON,DUVIVIER PIERRE,GAVARD OLLIVIER FRANCIS NICOLAS
申请号：
IN6942/CHENP/2013
公开号：
IN2013CN06942A
申请日:
2013.08.28
申请国别(地区):
IN
年份:
2014
代理人:
摘要：
Streptococcus pneumoniaeHaemophilus influenzaeNeisseria meningitidisStaphylococcus aureusEnterococcus faeciumEnterococcus faecalisSalmonellaStaphylococcus epidermidisThe present invention process a process for conjugation of an antigen comprising the steps of a) activating the antigen to form an activated antigen b) reacting the activated antigen and a carrier protein or a linker to form an imine group linking the activated antigen to the carrier protein or the linker and c) reducing the imine group using a reducing agent comprising a triacetoxyborohydride moeity to form a conjugated antigen or an antigen linker and optionally d) reacting the antigen linker with a carrier protein to form a conjugated antigen optionally wherein the antigen originates from Vi or optionally further comprising a step a ) of lyophilising the activated antigen and a carrier protein followed by reconstitution in DMSO or DMF.
